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adoptive cellular therapy act b16f10 cells  (ATCC)


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    Structured Review

    ATCC adoptive cellular therapy act b16f10 cells
    Day 7 established <t>B16F10</t> tumors were treated with no T cells, or 2 × 106 TRP T cells cultured in the presence of IP3R inhibitor 2-APB (IP3R TRP) or vehicle control. (A) Tumor growth and (B) survival were measured. Survival endpoints were considered tumor size ≥ 400mm2 with n = 5 mice per group. Experiment was repeated twice. (A) *P < 0.05, 2-tailed Student t test of Control TRP versus IP3RI TRP and (B) **P < 0.001, Log-rank test. (C) Representative flow cytometry plots and quantification of CD4/Vβ14+ splenocytes or (D) CD62L/CD44 phenotype of CD4+/Vβ14+ splenocytes,5 days post adoptive cell therapy. (E) Absolute numbers of CD4+/Vβ14+ T cells in spleens or of (F) CD4+ T cell in tumors 5 days post ACT. (C–F) Data points represent 3 mice per group, *P < 0.05, **P < 0.01 2-tailed Student t test.
    Adoptive Cellular Therapy Act B16f10 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adoptive+cellular+therapy+act+b16f10+cells/B16-F10/pmc05585019-83-0-9
    Average 99 stars, based on 8025 article reviews
    adoptive cellular therapy act b16f10 cells - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Modulation of Endoplasmic Reticulum Stress Controls CD4 + T Cell Activation and Anti-tumor Function"

    Article Title: Modulation of Endoplasmic Reticulum Stress Controls CD4 + T Cell Activation and Anti-tumor Function

    Journal: Cancer immunology research

    doi: 10.1158/2326-6066.CIR-17-0081

    Day 7 established B16F10 tumors were treated with no T cells, or 2 × 106 TRP T cells cultured in the presence of IP3R inhibitor 2-APB (IP3R TRP) or vehicle control. (A) Tumor growth and (B) survival were measured. Survival endpoints were considered tumor size ≥ 400mm2 with n = 5 mice per group. Experiment was repeated twice. (A) *P < 0.05, 2-tailed Student t test of Control TRP versus IP3RI TRP and (B) **P < 0.001, Log-rank test. (C) Representative flow cytometry plots and quantification of CD4/Vβ14+ splenocytes or (D) CD62L/CD44 phenotype of CD4+/Vβ14+ splenocytes,5 days post adoptive cell therapy. (E) Absolute numbers of CD4+/Vβ14+ T cells in spleens or of (F) CD4+ T cell in tumors 5 days post ACT. (C–F) Data points represent 3 mice per group, *P < 0.05, **P < 0.01 2-tailed Student t test.
    Figure Legend Snippet: Day 7 established B16F10 tumors were treated with no T cells, or 2 × 106 TRP T cells cultured in the presence of IP3R inhibitor 2-APB (IP3R TRP) or vehicle control. (A) Tumor growth and (B) survival were measured. Survival endpoints were considered tumor size ≥ 400mm2 with n = 5 mice per group. Experiment was repeated twice. (A) *P < 0.05, 2-tailed Student t test of Control TRP versus IP3RI TRP and (B) **P < 0.001, Log-rank test. (C) Representative flow cytometry plots and quantification of CD4/Vβ14+ splenocytes or (D) CD62L/CD44 phenotype of CD4+/Vβ14+ splenocytes,5 days post adoptive cell therapy. (E) Absolute numbers of CD4+/Vβ14+ T cells in spleens or of (F) CD4+ T cell in tumors 5 days post ACT. (C–F) Data points represent 3 mice per group, *P < 0.05, **P < 0.01 2-tailed Student t test.

    Techniques Used: Cell Culture, Control, Flow Cytometry

    Related Articles

    Cell Culture:

    Article Title: Modulation of Endoplasmic Reticulum Stress Controls CD4 + T Cell Activation and Anti-tumor Function
    Article Snippet: Lactate in cell culture media was measured with Lactic Acid kit (Sigma).Lactate in cell culture media was measured with Lactic Acid kit (Sigma).. Adoptive Cellular Therapy (ACT) B16F10 cells were obtained from ATCC and tested negative for mycoplasma in March 2015.. Cells were passaged three times prior to in vivo inoculation.Cells were passaged three times prior to in vivo inoculation.

    Control:

    Article Title: Modulation of Endoplasmic Reticulum Stress Controls CD4 + T Cell Activation and Anti-tumor Function
    Article Snippet: Lactate in cell culture media was measured with Lactic Acid kit (Sigma).Lactate in cell culture media was measured with Lactic Acid kit (Sigma).. Adoptive Cellular Therapy (ACT) B16F10 cells were obtained from ATCC and tested negative for mycoplasma in March 2015.. Cells were passaged three times prior to in vivo inoculation.Cells were passaged three times prior to in vivo inoculation.

    Flow Cytometry:

    Article Title: Modulation of Endoplasmic Reticulum Stress Controls CD4 + T Cell Activation and Anti-tumor Function
    Article Snippet: Lactate in cell culture media was measured with Lactic Acid kit (Sigma).Lactate in cell culture media was measured with Lactic Acid kit (Sigma).. Adoptive Cellular Therapy (ACT) B16F10 cells were obtained from ATCC and tested negative for mycoplasma in March 2015.. Cells were passaged three times prior to in vivo inoculation.Cells were passaged three times prior to in vivo inoculation.



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    adoptive cellular therapy act b16f10 cells  (ATCC)
    99
    ATCC adoptive cellular therapy act b16f10 cells
    Day 7 established <t>B16F10</t> tumors were treated with no T cells, or 2 × 106 TRP T cells cultured in the presence of IP3R inhibitor 2-APB (IP3R TRP) or vehicle control. (A) Tumor growth and (B) survival were measured. Survival endpoints were considered tumor size ≥ 400mm2 with n = 5 mice per group. Experiment was repeated twice. (A) *P < 0.05, 2-tailed Student t test of Control TRP versus IP3RI TRP and (B) **P < 0.001, Log-rank test. (C) Representative flow cytometry plots and quantification of CD4/Vβ14+ splenocytes or (D) CD62L/CD44 phenotype of CD4+/Vβ14+ splenocytes,5 days post adoptive cell therapy. (E) Absolute numbers of CD4+/Vβ14+ T cells in spleens or of (F) CD4+ T cell in tumors 5 days post ACT. (C–F) Data points represent 3 mice per group, *P < 0.05, **P < 0.01 2-tailed Student t test.
    Adoptive Cellular Therapy Act B16f10 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adoptive+cellular+therapy+act+b16f10+cells/B16-F10/pmc05585019-83-0-9
    Average 99 stars, based on 1 article reviews
    adoptive cellular therapy act b16f10 cells - by Bioz Stars, 2026-10
    99/100 stars
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    Day 7 established B16F10 tumors were treated with no T cells, or 2 × 106 TRP T cells cultured in the presence of IP3R inhibitor 2-APB (IP3R TRP) or vehicle control. (A) Tumor growth and (B) survival were measured. Survival endpoints were considered tumor size ≥ 400mm2 with n = 5 mice per group. Experiment was repeated twice. (A) *P < 0.05, 2-tailed Student t test of Control TRP versus IP3RI TRP and (B) **P < 0.001, Log-rank test. (C) Representative flow cytometry plots and quantification of CD4/Vβ14+ splenocytes or (D) CD62L/CD44 phenotype of CD4+/Vβ14+ splenocytes,5 days post adoptive cell therapy. (E) Absolute numbers of CD4+/Vβ14+ T cells in spleens or of (F) CD4+ T cell in tumors 5 days post ACT. (C–F) Data points represent 3 mice per group, *P < 0.05, **P < 0.01 2-tailed Student t test.

    Journal: Cancer immunology research

    Article Title: Modulation of Endoplasmic Reticulum Stress Controls CD4 + T Cell Activation and Anti-tumor Function

    doi: 10.1158/2326-6066.CIR-17-0081

    Figure Lengend Snippet: Day 7 established B16F10 tumors were treated with no T cells, or 2 × 106 TRP T cells cultured in the presence of IP3R inhibitor 2-APB (IP3R TRP) or vehicle control. (A) Tumor growth and (B) survival were measured. Survival endpoints were considered tumor size ≥ 400mm2 with n = 5 mice per group. Experiment was repeated twice. (A) *P < 0.05, 2-tailed Student t test of Control TRP versus IP3RI TRP and (B) **P < 0.001, Log-rank test. (C) Representative flow cytometry plots and quantification of CD4/Vβ14+ splenocytes or (D) CD62L/CD44 phenotype of CD4+/Vβ14+ splenocytes,5 days post adoptive cell therapy. (E) Absolute numbers of CD4+/Vβ14+ T cells in spleens or of (F) CD4+ T cell in tumors 5 days post ACT. (C–F) Data points represent 3 mice per group, *P < 0.05, **P < 0.01 2-tailed Student t test.

    Article Snippet: Adoptive Cellular Therapy (ACT) B16F10 cells were obtained from ATCC and tested negative for mycoplasma in March 2015.

    Techniques: Cell Culture, Control, Flow Cytometry